SKINPHAGE

To combat antibiotic-resistant bacteria, this project aims to develop a phage solution for use in localised bacterial infections (e.g., skin wounds). Wastewater samples were collected, and the “Escherichia phage vB_EcoM-fFi-Eco06” bacteriophage was selected and isolated. Its propagation and purification followed, as well as the extraction of its DNA, and the sequencing of its genome. The sequenced bacteriophage was propagated and purified, and a phage solution was prepared in which gauzes were soaked. These were tested against E. coli bacterial cultures to evaluate the POC: elimination of E. coli bacteria when in contact with the phage solution. The results validated the POC and the final product is a modular solution applicable to other types of bacteria.