Effect of m6A RNA Modification on Apoptosis in TRAIL-Treated MDA-MB-231 Breast Cancer Cells

We investigated the effect of m6A modification in TRAIL-treated MDA-MB-231 cells. An increase in m6A regulatory writer proteins (RBM15, WTAP, METTL3) was observed. This finding is contrary to the m6A change in the literature. The source of the change is due to a cell line-specific or ligand-specific feature that has not yet been discovered. The experiment to discover it is repeated with TNF-alpha, and if a decrease is observed, a ligand-specific feature is discovered. Hence a bioinformatic analysis in R studio with gene expression data obtained from GEO, the possible m6A-dependent TRAIL-induced apoptosis pathway and the mechanism of the cell developing resistance to TRAIL were established for the first time.