Determination of several biomarkers and pharmaceuticals in Riga’s wastewater using nanoflow liquid chromatography – Orbitrap mass spectrometry

The goal of this project was to observe changes in lifestyle habits and habits of pharmaceutical consumption in Riga, using the wastewater-based epidemiology (WBE) approach for determining several biomarkers and pharmaceuticals in wastewater using nanoflow liquid chromatography – Orbitrap mass spectrometry. The changes in concentrations of the analytes in the wastewater samples indicated patterns and the ability to evaluate the consumption of selected analytes. It was concluded that this method allows to determine the selected biomarkers and pharmaceuticals in wastewater at low concentrations using a simple dilute-and-shoot sample preparation procedure and modern sensitive analytical instrumentation, as well as to observe changes in the habits of consumption of the selected substances.